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| Registros recuperados: 33 | |
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| Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose, N-acetylglucosamine, and N-acetylgalactosamine, which form part of more than one type of oligosaccharide units. Autoproteolytic treatment mainly provided O-glycosidic type oligosaccharides, while a mixture of O- and N-glycosidic oligosaccharides was obtained in variable proportions when treated with trypsin, papain or pronase. The highest degree of peptide cleavage... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Carbohydrate; Glycoconjugate; Proteolysis; Human sperm; Peptide. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300013 |
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| Bergeron, Jean-pierre; Herbland, Alain. |
| Estimates of carbohydrate assimilation within the mesozooplankton compartment are a fundamental concern in oceanography because they give a general expression of the biologically-regulated incorporation of metabolic fuel produced from inorganic nutrients by phytoplankton in the sea. A new tool is proposed for the assessment of such processes. It is based on the measurement of the activity of an enzyme, pyruvate kinase (PK), on samples of the whole mesozooplankton community. Here, the results are presented of an early attempt at such an implementation in the Bay of Biscay (north-east Atlantic) in springtime. A main area situated in front of an estuary (Gironde) has been extensively studied-the same sampling grid was covered on three occasions (two 20 day... |
| Tipo: Text |
Palavras-chave: Bay of Biscay; Zooplankton; Phytoplankton; Pyruvate kinase; Carbohydrate. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-452.pdf |
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| Ricci,Adriana Patrícia; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Piedade,Sonia Maria de Stefano. |
| Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from 'Ponkan' mandarin (Citrus reticulata, Blanco), 'Cravo' mandarin (C. reticulata), 'Itaboraí' sweet orange (C. sinensis L. Osbeck.), 'Valencia' sweet orange (C. sinensis) and 'Kinnow' mandarin (C. nobilis Loureiro x C. deliciosa Tenore). The culture medium used was MT supplemented with sucrose, galactose, glucose, maltose or lactose with the following concentrations of 18, 37, 75, 110, and 150 mM. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Callus; Carbohydrate; Citrus; Somatic embryo. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100005 |
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| Vieira,L.A.P.; Freitas,A.L.P.; Feitosa,J.P.A.; Silva,D.C.; Viana,G.S.B.. |
| Bryothamnion seaforthii, a red alga common to the Northeastern coast of Brazil, was used to prepare the protein fraction F0/60 by ammonium sulfate precipitation. The chromatography of F0/60 on DEAE-Sephadel column resulted in two lectin fractions, PI and PII, which have antinociceptive properties in rodents. We determined the antinociceptive activity of the PII fraction and of a carbohydrate-containing fraction (CF) in mice. The CF was prepared from the dried algae, after digestion with 100 mM sodium acetate, pH 6.0, containing 5 mM cysteine, EDTA and 0.4% papain, at 60ºC. A 10% cetylpyridinium chloride was added to the filtrate, and the precipitate was dissolved with 2 M NaCl:ethanol (100:15, v/v) followed by the carbohydrate precipitation with ethanol.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algae; Antinociceptive activity; Carbohydrate; Bryothamnion seaforthii. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700017 |
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| Dantas,Bárbara França; Pereira,Maiane Santos; Ribeiro,Luciana de Sá; Maia,Joselanne Luiza Trajano; Silva,Davi José; Duenhas,Luiza Helena; Lima,Maria Auxiliadora Coelho de; Bassoi,Luis Henrique. |
| The guava (Psidium guajava L.) cv. Paluma has been cultivated in São Francisco Valley, Northeastern of Brazil, for in natura consumption and processing purposes. In spite of its importance, there are few scientific knowledge regarding guava physiology, nutrition, irrigation and fertigation. The objective of this work was to evaluate the effect of weather conditions and different concentrations of N and K applied by fertigation in foliar contents of reducing sugars, total soluble sugars, starch, sucrose, amino acids, and proteins. The field experiment was carried out at Bebedouro Experimental Field and the biochemical evaluations at the Laboratory of Seed and Plant Physiology, both located at Embrapa Semi-Árido, Petrolina-PE. The doses of 200 g N and 100 g... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psidium guajava; Primary metabolism; Carbohydrate. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452007000200026 |
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| Hall,Mary Beth. |
| Carbohydrates can provide up to 80% of the dry matter in animal diets, yet their specific evaluation for research and diet formulation is only now becoming a focus in the animal sciences. Partitioning of dietary carbohydrates for nutritional purposes should reflect differences in digestion and fermentation characteristics and effects on animal performance. Key challenges to designating nutritionally important carbohydrate fractions include classifying the carbohydrates in terms of nutritional characteristics, and selecting analytical methods that describe the desired fraction. The relative lack of information on digestion characteristics of various carbohydrates and their interactions with other fractions in diets means that fractions will not soon be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Analysis; Carbohydrate; Fiber; Starch; Sugars. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007001000032 |
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| Rocha-de-Souza,Cláudio M.; Hirata-Jr,Raphael; Mattos-Guaraldi,Ana L.; Freitas-Almeida,Angela C.; Andrade,Arnaldo F. B.. |
| The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus (EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding lectin) in AeC398... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas caviae; Lectin; Carbohydrate; Cell surface. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200003 |
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| Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M. |
| To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is... |
| Tipo: Text |
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf |
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| Registros recuperados: 33 | |
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